Trehalose as Cryoprotectant for the Freeze Preservation of Carrot and Tobacco Cells
نویسندگان
چکیده
منابع مشابه
Trehalose as cryoprotectant for the freeze preservation of carrot and tobacco cells.
Suspension cultures of carrot (Daucus carota, line C1), tobacco (Nicotiana tabacum, line TX1), and Nicotiana plumbaginifolia (line NP) were frozen under controlled conditions with trehalose as the sole cryoprotectant. Maximal post-thaw viability (71-74%), measured by phenosafranin dye exclusion, was obtained with the C1 cells following a 24hour pretreatment with 5 or 10% trehalose and with 40% ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1985
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.78.2.430